Journal: BMC Microbiology

Article Title: Reproductive tract microbiota dysbiosis in ovarian endometrioma and adenomyosis: multi-site 16S rRNA profiling and functional impact of key bacterial species on human endometrial stromal cells

doi: 10.1186/s12866-025-04412-7

Figure Lengend Snippet: Validation of key differentially expressed genes (DEGs) at mRNA and protein levels. A-B qRT-PCR validation of relative mRNA expression levels for selected genes (CEBPB, SERPINE1, S100A1, CKS1B, ADH1B, MYOM1, PTGFR, ACLY) in T-HESCs comparing AM vs. NC and CC vs. NC co-culture conditions. Expression normalized to GAPDH. C-D ELISA validation of protein concentrations for corresponding key molecules in cell culture supernatants, comparing AM vs. NC and CC vs. NC co-culture conditions. Data presented as mean ± SD or similar; significance indicated ( P < 0.05)

Article Snippet: Kits used included those for: ADH1B (MyBioSource, Cat. No. MBS4503934); MYOM1 (Thermo Fisher Scientific, Cat. No. PIPA5100626; or Proteintech, Cat. No. 22084-1-AP); PTGFR (ABclonal, Cat. No. RK07240; or MyBioSource, Cat. No. MBS9714736); ACLY (RayBiotech, Cat. No. ELH-ACLY; Biomatik, Cat. No. EKF60626 ; BPS Bioscience, Cat. No. 79904; or ABclonal, Cat. No. RK12483); CEBPB (Antibodies-online, Cat. No. ABIN989871); SERPINE1 (Proteintech, Cat. No. KE00109; MyBioSource, Cat. No. MBS175945; R&D Systems, Cat. No. DSE100; ABclonal, Cat. No. RK00133; or Sino Biological, Cat. No. KIT10296 ); S100A1 (Thermo Fisher Scientific, Cat. No. EH402RB; RayBiotech, Cat. No. ELH-S100A1; or MyBioSource, Cat. No. MBS4503934); and CKS1B (MyBioSource, Cat. No. MBS7207214; or Biomatik, Cat. No. EKA50789 ).

Techniques: Biomarker Discovery, Quantitative RT-PCR, Expressing, Co-Culture Assay, Enzyme-linked Immunosorbent Assay, Cell Culture

Journal: Cell Reports Medicine

Article Title: A spatially resolved transcriptome landscape during thyroid cancer progression

doi: 10.1016/j.xcrm.2025.102043

Figure Lengend Snippet: Cell-cell communications in thyroid cancer (A) Bubble plot illustrating the spatial correlations of dysregulated ligand-receptor pairs between PTC, LPTC, or ATC and PT samples in the ST data. (B and C) Bar plots showing fold changes in ligand (B) and receptor (C) expression in PTC, LPTC, and ATC samples compared to PT samples. (D) Spatial feature plots highlighting SERPINE1 (ligand) and PLAUR (receptor) expression in representative samples (PT-2, PTC-2, LPTC-2, and ATC-3). (E and F) Expression levels of SERPINE1 (E) and PLAUR (F) across various cell types. (G) SERPINE1 expression within fibroblasts, with the inset indicating enrichment of fibroblast subpopulations stratified by SERPINE1 expression levels. (H) PLAUR expression within macrophages, with the inset showing enrichment of macrophage subpopulations stratified by PLAUR expression levels. (I) Multiplex immunofluorescence staining for Fib-12 fibroblast markers ( CXCL8 , CXCL1 , and SERPINE1 ) and Mac-11 macrophages ( CXCL12 , STAB1 , and PLAUR ) in PTC, LPTC, and ATC samples. Scale bar: 40 μm. (J) Boxplot comparing SERPINE1 expression in cancer ( n = 58) versus paired NAT ( n = 58) samples from the TCGA thyroid carcinoma (THCA) cohort. (K) Kaplan-Meier survival curve from the TCGA THCA cohort ( n = 510) showing patient survival based on median SERPINE1 expression levels. See also Figure S6 and Tables S5 and .

Article Snippet: Anti-human SERPINE1 antibody, clone 1H4A5 , Proteintech , Cat# 66261-1-Ig; RRID: AB_2881648.

Techniques: Expressing, Multiplex Assay, Immunofluorescence, Staining

Journal: Cell Reports Medicine

Article Title: A spatially resolved transcriptome landscape during thyroid cancer progression

doi: 10.1016/j.xcrm.2025.102043

Figure Lengend Snippet:

Article Snippet: Anti-human SERPINE1 antibody, clone 1H4A5 , Proteintech , Cat# 66261-1-Ig; RRID: AB_2881648.

Techniques: Recombinant, Software